Journal: Analytical biochemistry

Article Title: A secreted protein microarray platform for extracellular protein interaction discovery.

doi: 10.1016/j.ab.2011.09.017

Figure Lengend Snippet: Fig.2. Specific binding of Fc fusion–protein A microbead complexes. Bar plots displaying the background subtracted fluorescent signal normalized against the average background subtracted fluorescent signal for the whole slide are shown. The top ranking interactors are shown for screens of CD2-Fc (A), CD200R1-Fc (B), TIGIT-Fc (C), JAM3- Fc (D), and CD160-Fc (E) baits. Black bars represent known interactions, and gray bars represent background or unexpected binding.

Article Snippet: The relative immobilization level for each Fc-, His/Gln-, and His-tagged protein was determined by probing two replicate slides with a Cy5-labeled anti-Fc antibody (Jackson ImmunoResearch, cat. no. 109-176-170), a Cy5-labeled anti-His/Gln antibody (Genentech), or a multivalent anti-His microbead (Miltenyi Biotec, cat. no. 130-091-124) detected with Cy5-labeled goat anti-mouse F(ab0)2 (Jackson ImmunoResearch, cat. no. 115-176-072).

Techniques: Binding Assay

Journal: Experimental Biology and Medicine

Article Title: Maternal diet-induced hypercholanemia alters gut microbiota and metabolome in adult female Western diet-fed offspring

doi: 10.3389/ebm.2026.10810

Figure Lengend Snippet: Individual bile acid species present in cecal content determined by bile acid profiling UHPLC-MS/MS. (A) Pregnant (P) and non-pregnant (NP) mice fed normal chow (NC) or cholic acid (CA)-supplemented diet. *p < 0.05, **p < 0.01, ***p < 0.001 as determined by Student’s t-test following Pareto scaling and model fitting with SIMCA (full statistical information shown in ) comparing two groups to test the following effects: # Pregnancy effect in NC-fed females (P NC vs. NP NC); *CA effect in non-pregnant females (NP CA vs. NP NC); § CA effect in pregnant female (P CA versus P NC). n = 4–6. (B) Female offspring from NC- or CA-fed dams challenged with Western diet (WD) or NC. *p < 0.05 as determined by Student’s t-test following Pareto scaling and model fitting with SIMCA (full statistical information shown in . n = 6. Box plots show mean and range. Abbreviations: CA, cholic acid; TCA, taurocholic acid; β-MCA, β-muricholic acid; ω-MCA, ω-muricholic acid; TMCAs, Tauro-muricholic acids; DCA, deoxycholic acid; LCA, lithocholic acid; HDCA, hyodeoxycholic acid; DHCA, dehydrocholic acid.

Article Snippet: Orthogonal projection to latent structures discriminant analysis (OPLS-DA) were employed to analyze UHPLC-MS data in a multivariate setting, using the SIMCA package (v.13.0.2; Umetrics, Umea, Sweden).

Techniques: Tandem Mass Spectroscopy, Western Blot